Papillomavírus kv g

Instituto Oncologico Luis Razetti Rövid összefoglaló 1.

papillomavírus kv g

Hypothesis: If high risk genotypes of human papillomavirus HPV is the cause of cervical cancer worldwide, the genotypes associated with cervical cancer in Venezuela would be the same types found in other countries. Specific objectives: To establish the presence of HPV in cervical cancer patients in Venezuela by geographic distribution.

papillomavírus kv g

To establish which HPV types are linked with cervical cancer in Venezuela by geographic distribution. Részletes leírás In Venezuela, cervical cancer is the second leading cause of cancer mortality in women. In developed countries, although it is not the first cause of cancer mortality, occupies a remarkable place in the neoplastic pathology Cannistra, ; Dey, Human Papilloma Virus HPV is involved in the development of cervical cancer, specially oncogenic types 16 and 18 Koskela, ; Markowska, To date, it is still unknown which HPV types are more frequent in Venezuela.

Several studies made in this country concluded that HPV 16 is the predominant type in cervical pathology GraterolCorrentiMuñozbut there has not been performed a HVP genotyping to detect all the types involved in patients with cervical cancer.

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Neither has been establish which HPV types are more frequently involved in genital warts in Venezuela. The goal of this investigation is perform a genotypification of HPV in cervical cancer and cervical intraepithelial neoplasia 2 and 3 in Venezuela to determine which types are more frequently related to this disease papillomavírus kv g our country.

papillomavírus kv g

Collection of cervical samples: 1. Previous ethics committee approval Instituto Venezolano de Investigaciones Cientificas and Instituto de Oncología Luis Razettiwe are going to collect paraffin blocks of patients with histological diagnosis of CIN or Stage I cervical cancer. Inclusion criteria: 1.

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Properly blocks identification in order to obtain personal and clinical patient information. Molecular biology procedures: 1.

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Histologically handling of tissue: previous identification of pathologic lesions as described in inclusion criteria by an expert pathologist sample with lesion but without necrosisthe paraffin block will be cut into 5 to 7 µm slides with new blades and gloves for each cut.

It will be obtain 1 slides that will be distributed as follows: one sample will be send to Laboratorio de Virología Molecular del Instituto Venezolano de Investigaciones Científicas; the remained sample will be kept for further evaluation if is necessary.

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Each slide will be placed in a proper container and will be identified by a serial number in order to preserve papillomavírus kv g intimacy and avoid biased results. Deparaffinisation as described in Coombs Tissue digestion, DNA extraction and precipitation as described in Gravitt The results will be correlated with clinical information.

HPV and Cancer – An Introduction

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